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KMID : 0380020210360010042
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Korean Journal of Biotechnology and Bioengineering
2021 Volume.36 No. 1 p.42 ~ p.48
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Melanogenesis Inhibitory Effects of Phryma leptostachya Callus using Biorenovation in B16F10 Melanoma Cells
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Lee Kyung-Mi
Park Tae-Jin
Jie Eun-Yee
Kim Suk-Weon
Han Dong-Hwan
Kim Seung-Young
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Abstract
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Bio-renovation is the microorganism-assisted conversion of the structure and improvement of biological efficacies of natural products and synthetic compounds. In this study, we investigated the effect of plant callus on anti-melanogenic effect and compared the activity changes of bio-renovated callus. Phryma leptostachya bio-renovation products at the concentrations of 100, 200, 400 μg/mL suppressed tyrosinase activity and the expression of melanogensis-related protein such as tyrosinase-related protein-1 (TRP-1), tyrosinase-related protein-2 (TRP-2) and microphthalmia-associated transcription factor (MITF) without a loss of cell viability in α-melanocyte-stimulating hormone (α-MSH)-stimulated B16F10 melanoma cells relative to its substrate. Moreover, cell viabilities of PLBR increased over the tested concentrations compared to its substrate (PL). PLBR at 400 μg/mL inhibited the α-MSH-induced melanogensis effect to the level of non-treated control. These results indicate that the structure of various compounds contained in Phryma leptostachya (PL) were modified through a bio-renovation technique and which conferred anti-melanogenic properties to PL.
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KEYWORD
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Phryma leptostachya, Bio-renovation, Tyrosinase activity, Whitening agent, MITF
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